AN EXPERIMENTAL STUDY ON ALBINO RATS TO COMPARE THE HEPATOPROTECTIVE EFFECT OF ORALLY ADMINISTERED DL-METHIONINE AND N-ACETYLCYSTEINE ON THE LIVER INJURY CAUSED BY POSITIVE CONTROL DRUG DICLOFENAC SODIUM

Introduction: Liver, main organ for biotransformation of drugs, during which most the drugs are lipophilic in nature made hydrophilic by biochemical processes liver cells resulting into water-soluble products get excreted urine or bile. Adverse drug reactions (ADRs) pertaining to liver, can be mainly two types, predictable dose dependent and idiosyncratic. To counter oxidative stress cellular damage, several antioxidant enzymes have been developed including superoxide dismutase (SOD), Glutathione peroxidase catalase, that detoxify reactive oxygen species contained endogenous antioxidants scavenge free radicals reduces damage glutathione plays a major role. Synthesis is regulated at substrate level cysteine, synthesized from homocysteine via trans-sulfuration pathway N-Acetylcysteine used as specific treatment Paracetamol overdose, serves prodrug L-Cysteine, precursor Glutathione. L-methionine, L-cysteine, considered activity, found well. Methionine particularly important opposing toxicity generated various toxins. Hence, supplementation same has proposed greater role reducing toxic effect on liver. The aim was study comparative hepatoprotective orally administered DL-Methionine injury induced positive control Diclofenac Sodium. Materials Methods: healthy albino rats were grouped experimental study, groups (n=6). After overnight fasting, belonging Group I Vehicle treated (n=6) Distilled Water 10 ml/kg II V single oral concomitantly with sodium (96 mg/kg & 240 p.o.) (700 p.o. 1400 respectively. VI VII 450 p.o., Serum samples each collected after 24-hours post-treatment, effects Sodium through Haemato-biochemical changes. Results: vehicle-treated group showed no significant alteration function tests morphology. On concomitant administration 700 96 it observed there occurred reduction (p < 0.001) serum SGOT SGPT levels compared group. their statistically decrease when between agents. mg/kg, levels. It <0.05) levels, mg/kg. However, both doses DL-Methionine, changes Total Bilirubin, Alkaline Phosphatase Gamma-Glutamyl Transpeptidase (GGTP) Conclusion: We effectiveness against Diclofenac-induced hepatotoxicity, where we observed, transaminases elevated due hepatotoxic diclofenac sodium. much difference N-Acetylcysteine. In summary, our present reinforced concept hepatotoxicity protected administering NAC even cases non-paracetamol injury.